Caspase 3 (Pro & Active Form) Antibody

SKU: orb2309393

Description

Caspase 3 (Pro & Active Form) Antibody

Research Area

,Apoptosis|Autophagy|Cardiovascular|Colon Cancer|Complement System|Cytokine Signaling|Immunology|Lung Cancer|MAPK Signaling|Mitochondria Marker|Signal Transduction

Images & Validation

• Tested Applications: IHC
• Dilution Range: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: HL60 or Jurkat cells. Human liver, spleen, heart, liver or kidney.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: CASP3/8446
• Immunogen: Recombinant fragment (around aa1-200) of human CASP3 protein (exact sequence is proprietary)
• Target: CASP3
• Source/Expression System: Bioreactor
• Molecular Weight: 32kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

APOPAIN; Apoptosis related cysteine peptidase; CASP3; Caspase3; CPP32; CPP32B; cysteine protease CPP32; PARP cleavage protease; Procaspase3; SREBP cleavage activity 1 (SCA1); Yama

Quick Database Links

Gene Symbol

CASP3

UniProt

P42574

NCBI Gene ID

836