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Description
Research Area
,Product Categories/Antibodies,Apoptosis / Caspase
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot 1:500 - 1:2000 Immunofluorescence 1:200 – 1:500 Immunohistochemistry (Paraffin) 1:200 – 1:300 |
| Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / encompassing a sequence within the center region |
| Target | Caspase-8 Cleaved |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
|---|---|
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Caspase 8 antibody - at 1/1000 dilution. Lysates at 50 μg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294354 overnight at 4°C.
Caspase 8 antibody - at 1/1000 dilution, Lysates at 50 μg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294354 overnight at 4°C.
immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Caspase 8 with orb1294354 at 1/200.
Customer feedback Image. Immunohistochemical of Paraffin-embedded. Sample: Human colon cancer. Caspase 8 antibody (orb1294354): 1-200.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294354 Anti-Caspase 8 antibody (red) at 1:150 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Quick Database Links
Gene Symbol
Caspase-8 Cleaved
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
Caspase-8 Cleaved (orb1294354)
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