Cat IgG Fc Antibody Alkaline Phosphatase Conjugated

SKU: orb346873

Description

Cat IgG F(c) antibody (Alkaline Phosphatase)

Research Area

Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: DOT, ELISA, IHC, WB
• Dilution range: ELISA: 1:4,000 - 1:16,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Reactivity: Feline
• Application Notes:
  Anti-Cat IgG F(c) Alkaline Phosphatase conjugate has been tested by ELISA and dot blot. This product is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Cat IgG F(c) fragment
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Cat IgG, Cat IgG F(c) and Cat Serum. No reaction was observed against Cat IgG F(ab).
• Conjugation: AP

Storage & Handling

• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Disclaimer: For research use only

Alternative Names

Rabbit Anti-Cat IgG F(c) Alkaline Phosphatase Conjugated Antibody, Rabbit Anti-Cat IgG F(c) Fragment Antibody Alk Phos conjugation

Dot Blot of Rabbit anti-Cat IgG Fc Antibody Alkaline Phosphatase Conjugated. Antigen: Cat IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Rabbit anti-Cat IgG Fc Antibody Alkaline Phosphatase Conjugated at 1:1000 for 60 min at RT. Block: orb348637 for 1 HR at RT.