Cattle PRL(Prolactin) ELISA Kit

SKU: orb2667455

Description

Cattle PRL(Prolactin) ELISA Kit

Research Area

,Endocrinology;Reproductive science;Genetic science;Hormone metabolism;

Images & Validation

• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle PRL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle PRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle PRL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle PRL in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Sample Types: serum, plasma and other biological fluids
• Range: 0.32-20 ng/mL
• Sensitivity: 1.39 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

LTH; Luteotropic Hormone

Quick Database Links

UniProt

P01239