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Description
Research Area
Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Infectious Diseases,Cell Signaling | Epigenetics and Nuclear Signaling | Chromatin Modifying Enzymes | Transcription | Stem Cells | Signaling Pathways | Wnt / Beta Catenin Pathway | Cancer | Cancer Metabolism | Cancer Lipid and Lipoprotein Metabolism | Cancer | Cancer Metabolism | Response to Hypoxia
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | WB (1:1000); IHC (1:50) |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide from the mid-protein of Human CBP (aa. 2100-2200) |
| Target | CBP |
| Molecular Weight | 265 kDa |
| Conjugation | HRP |
Storage & Handling
−| Storage | Conjugated antibodies should be stored according to the product label |
|---|---|
| Buffer/Preservatives | 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−RSTS antibody, CBP_HUMAN antibody, Cyclic AMP responsive enhancer binding protein antibody, Crebbp antibody, CREB-binding protein antibody, Rubinstein Taybi syndrome antibody, KAT3A antibody, EC:2.3.1.48 antibody, CREBBP antibody, CBP antibody, RTS antibody, CREB binding protein antibody
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Immunohistochemistry analysis using Rabbit Anti-CBP Polyclonal Antibody. Tissue: Breast Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-CBP Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~265.3 kDa CBP protein using Rabbit Anti-CBP Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CBP Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~265.3 kDa.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001073315.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CBP Antibody: HRP (orb535293)
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