CCND3 Antibody (C-term S274)

SKU: orb107017

Description

Rabbit polyclonal to CCND3. Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Primary Antibodies;Cancer;Cell Biology;Anti-Wnt-Pathway (plus Lgr5) Antibodies;Jak-STAT signaling KEGG

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:1000
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB43029
• Immunogen: Synthetic Peptide
• Target: CCND3
• Molecular Weight: 32520
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CCND3 antibody, anti G1/S-specific cyclin-D3 antibody

Formalin-fixed and paraffin-embedded H.skin tissue reacted with CCND3 Antibody (C-term S274).

CCND3 Antibody (C-term S274) western blot analysis in MDA-MB231 cell line lysates (35 ug/lane). This demonstrates the CCND3 antibody detected the CCND3 protein (arrow).

CCND3 Antibody (C-term S274) western blot analysis in mouse NIH-3T3 cell line lysates (35 ug/lane). This demonstrates the CCND3 antibody detected the CCND3 protein (arrow).

Quick Database Links

Gene Symbol

CCND3

UniProt

P30281

RefSeq (Protein):NP_001751.1, NP_001129597.1, NP_001129489.1, NP_001129598.1