CD11b Antibody (N-term)

SKU: orb28586

Description

Rabbit polyclonal antibody to CD11b

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Neuroscience,Immunology,Epigenetics,Primary Antibodies;Immunology;Neuroscience;Signal Transduction;Inflammation mediated by chemokine and cytokine signaling;Integrin Antibodies

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:50-100, FACS: 1:10-50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB17991
• Immunogen: Synthetic Peptide
• Target: ITGAM
• Molecular Weight: 127179
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Antigen CD11b p170 antibody, anti CD11 antigen like family member B antibody, anti CD11 antigen-like family member B antibody, anti CD11b antibody, anti CD11b antigen antibody, anti CD11b/CD18 antibody, anti CD49d antibody, anti Cell surface glycoprotein MAC 1 alpha subunit antibody, anti Cell surface glycoprotein MAC-1 subunit alpha antibody, anti Cell Surface Glycoprotein MAC1 Alpha Subunit antibody, anti Complement component 3 receptor 3 subunit antibody, anti Complement Component Receptor 3 Alpha antibody, anti Complement receptor type 3, alpha subunit antibody, anti CR 3 alpha chain antibody, anti CR-3 alpha chain antibody, anti CR3 Alpha Chain antibody, anti CR3 antibody, anti CR3A antibody, anti F730045J24Rik antibody, anti Integrin Alpha M antibody, anti Integrin alpha M chain antibody, anti integrin alpha M complement component 3 receptor 3 subunit antibody, anti Integrin alpha-M antibody, anti Integrin beta 2 alpha subunit antibody, anti ITGAM antibody, anti Leukocyte adhesion receptor MO1 antibody, anti MAC 1 antibody, anti Mac-1a antibody, anti MAC1 antibody, anti Mac1, alpha subunit antibody, anti MAC1A antibody, anti Macrophage antigen alpha polypeptide antibody, anti Mo1, alpha subunit antibody, anti Neutrophil adherence receptor alpha M subunit antibody, anti Neutrophil adherence receptor antibody

Anti-CD11b Antibody (N-term) at 1:2000 dilution + human spleen lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 127 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-CD11b Antibody (N-term) at 1:500 dilution + human spleen lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 127 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

CD11b Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

CD11b Antibody (N-term) western blot analysis in A549, K562 cell line lysates (35 ug/lane). This demonstrates the CD11b antibody detected the CD11b protein (arrow).

Staining D11b in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.

Staining D11b in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.

Staining D11b in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.

Quick Database Links

Gene Symbol

ITGAM

UniProt

P11215

RefSeq (Protein):NP_000623.2, NP_001139280.1