CD133 Antibody / PROM1 (Extracellular Region)

SKU: orb2636883

Description

CD133, also known as PROML1 or Prominin, is a stem cell antigen that may be useful for the selection and expansion of hematopoietic cells and may be used as a positive marker for the characterization of trophoblast cell lines. The human CD133 gene maps to chromosome 4p15.32 and encodes an 865 amino acid protein. The CD133 gene codes for a pentaspantransmembrane glycoprotein that is expressed on primitive hematopoietic stem, progenitor, retinoblastoma, hemangioblasts and neural stem cells and developing epithelium. The 5-TM structure includes an extracellular N-terminus, two short intra- cellular loops, two large extracellular loops and an intracellular C-terminus. CD133 is a candidate gene for retinal proteins that are targeted to plasma membrane protrusions. These retinal proteins, including CD133, may influence the shedding of photoreceptive membranes from the terminal end of the outer segments of vertebrate photoreceptors, where they are phagocytosed by the retinal pigment epithelium, and represent candidates for inherited retinal degenerations.

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the CD133 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: PROM/6316
• Immunogen: A chimeric protein composed of a portion of amino acids 180-380 and 612-765 (extracelluar regions) was used as the immunogen for the CD133 antibody. This Mab recognizes both glycosylated and non-glycosylated extracellular domains of CD133.
• Purity: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This CD133 antibody is available for research use only.
• Disclaimer: For research use only

Quick Database Links

UniProt

O43490