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Description
Research Area
,Apoptosis|B Cell Markers|Cardiovascular|Dendritic Cell Marker|Hematopoietic Stem Cells|Immunology|Infectious Disease|MAPK Signaling|Mesenchymal Stem Cell Differentiation
Images & Validation
−
| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | Western Blot (1-2ug/ml);Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone No. | LPSR/2397 |
| Immunogen | Recombinant fragment of human CD14 protein (around aa 25-148) (exact sequence is proprietary) |
| Target | CD14 |
| Source/Expression System | HEK293 cells |
| Molecular Weight | 55kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Concentration | Purified Ab WITHOUT BSA and Azide at 1.0mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Lipopolysaccharide receptor; LPS-R; LPSR; Mo2; Monocyte Differentiation Antigen 14; Myeloid cell-specific leucine-rich glycoprotein
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry