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Description
Research Area
Product Categories/Antibodies/Primary Antibodies,Product Categories/Small Molecules,Product Categories/Antibodies
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IHC-P |
|---|---|
| Dilution Range | Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human FucT-IV. AA range:22-71 |
| Molecular Weight | 46kD |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti-FUT4 antibody, anti-ELFT antibody, anti-FCT3A antibody, anti-Alpha-(1 antibody, anti-3)-fucosyltransferase antibody, anti-ELAM-1 ligand fucosyltransferase antibody, anti-Fucosyltransferase 4 antibody, anti-Fucosyltransferase IV antibody, anti-Fuc-TIV antibody, anti-FucT-IV antibody, anti-Galactoside 3-L-fucosyltransferase antibody
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Fluorescence multiplex immunohistochemical analysis of normal human gastric cancer tissue (formalin-fixed paraffin-embedded section).The section was incubated in 3 rounds of staining; in the order of CD15 YT0726 (1/50 dilution), CD11b YT5923 (1/50 dilution), CD14 YM6145 (1/50 dilution), each using a separate fluorescent tyramide signal amplification system.Sodium citrate antigen retrieval (Immunoway YS0002, 30 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity.DAPI (dark blue) was used as a nuclear counter stain.
Immunohistochemistry analysis of FucT-IV antibody in paraffin-embedded human breast carcinoma tissue.
Protocol Information
IHC-P
Immunohistochemistry Paraffin
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CD15 rabbit pAb (orb768288)
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