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Description
Research Area
,Immunology
Images & Validation
−Item 1 of 2
| Tested Applications | IF, WB |
|---|---|
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 50-164 of human CD247 (NP_932170.1). |
| Target | CD247 |
| Molecular Weight | Observed: 17kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD247, CD247 molecule, CD3-ZETA, CD3H, CD3Q, CD3Z, T3Z, TCRZ, CD247 antigen, zeta subunit, CD3Z antigen, zeta polypeptide (TiT3 complex), CD3zeta chain, T-cell antigen receptor complex, zeta subunit of CD3, T-cell receptor T3 zeta chain, T-cell surface gl
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Western blot analysis of extracts of mouse skeletal muscle, using CD247 antibody (orb1258313) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of A549 cells using CD247 antibody (orb1258313). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
CD247
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
CD247 Antibody (orb1258313)
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