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Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized rh B7-DC / PD-L2 / CD273 at 1 μg/mL (100 μl/well) can bind rhPDCD-1 with a linear range of 15 - 500 ng/mL. |
| Target | PDCD1LG2 |
| Tag | His Tag |
| MW | 23.4 kDa |
| Purity | > 95% as determined by SDS-PAGE. |
| Protein Sequence | Leu 20 - Pro 219 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human PDL2 Protein, His Tag [orb757453]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 23.4 kDa after removal of the signal peptide.The apparent molecular mass of PDL2-His is approximately 35-55 kDa due to glycosylation.
Mammalian
10 μg, 100 μg, 50 μgRecombinant human PDCD1LG2 protein, C-His-Avi (HEK293) [orb1516350]
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 40-52 kDa based on Tris-Bis PAGE result. kDa
100 μgCD273 Recombinant Protein [orb1227912]
WB
> 95% as determined by SDS-PAGE more than 90% measured by SEC-HPLC.
49.2 kDa
HEK293 cells
0.1 mgPD-L2 Recombinant Protein [orb1227729]
ELISA, WB
> 95% as determined by reduced SDS-PAGE.Less than 1.0 EU per ug by the LAL method.
48.9 kDa
HEK293 cells
0.1 mgCD273 Recombinant Protein [orb1227604]
WB
> 95% as determined by SDS-PAGE.
23.4 kDa
HEK293 cells
0.1 mg

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