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Description
Research Area
Images & Validation
−| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:100-1000, IHC-P: 1:100-500 |
| Reactivity | Guinea pig, Human, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human CD28. Please contact us for the exact immunogen sequence. The peptide is available as orb13952. |
| Target | CD28 |
| Molecular Weight | 25 kDa |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Immunohistochemical staining of paraffin embedded guinea pig bone tissue using anti-CD28 (2.5 ug/ml)

Immunohistochemical staining of guinea pig bone marrow tissue using anti-CD28 (2.5 ug/ml)

IHC-P staining of rat thyroid tissue using anti-CD28 (2.5 ug/ml)

IHC-P image of rat thymus tissue using anti-CD28 (2.5 ug/ml)

WB analysis of rat lymph node (lane 1), rat thymus (lane 2), 293t cells (lane 3), mouse spleen (lane 4), rat bone marrow (lane 5); rat thyroid (lane 6) using anti-CD28 (1 ug/ml)

Immunohistochemical staining of rat lymph node tissue using CD28 antibody (2.5 ug/ml)

Immunohistochemical staining of rat lymph node tissue using CD28 antibody (2.5 ug/ml)
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Protocol Information
CD28 antibody (orb389337)
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