CD3 epsilon Antibody

SKU: orb2643993

Description

Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. This MAb has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection.

Images & Validation

• Tested Applications: FACS, Functional Studies, ICC, IF
• Dilution Range: Flow cytometry: 1-2ug/million cells,Immunohistochemistry (acetone fixed frozen sections),Immunofluorescence: 1-2ug/ml,Functional Studies (order BSA/azide-free format)
• Reactivity: Human
• Application Notes:
  Optimal dilution of the antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: OKT3
• Immunogen: Human PBMC were used as the immunogen for the CD3e antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This CD3e antibody is available for research use only.
• Disclaimer: For research use only

Flow cytometry staining of lymphocyte-gated human PBM cells with CF640R-labeled CD3e antibody (clone OKT3). Gray=unstained, Red=CF640R-CD3e antibody.

SDS-PAGE analysis of purified, BSA-free CD3e antibody (clone OKT3) as confirmation of integrity and purity.

Quick Database Links

UniProt

P07766