CD3 epsilon Antibody

SKU: orb2644093

Description

CD3 chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. The 145-2C11 antibody is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vivo T cell depletion.

Images & Validation

• Tested Applications: FACS, Functional Studies, IF
• Dilution Range: Functional Studies (order BSA/azide-free format),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Reactivity: Mouse
• Application Notes:
  Optimal dilution of the antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Armenian Hamster IgG, kappa
• Clone No.: 145-2C11
• Immunogen: The H2Kb-specific mouse cytotoxic T lymphocyte clone BM10 37 was used as the immunogen for the CD3e antibody.
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This CD3e antibody is available for research use only.
• Disclaimer: For research use only

SDS-PAGE analysis of purified, BSA-free CD3e antibody (clone 145-2C11) as confirmation of integrity and purity.

Quick Database Links

UniProt

P07766