CD3 epsilon Antibody

SKU: orb606533

Description

This antibody recognizes the epsilon chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Immunology,Epigenetics

Images & Validation

• Tested Applications: ELISA, FACS, IF, IHC
• Dilution Range: ELISA: 2-4ug/ml (order BSA/azide-free format),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (Acetone-fixed frozen tissues): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the CD3e antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: UCHT1
• Immunogen: Human infant thymocytes and peripheral blood lymphocytes from a Sezary Syndrome donor were used as the immunogen for the CD3e antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store the CD3e antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This CD3e antibody is available for research use only.
• Disclaimer: For research use only

Immunofluorescent staining of human Jurkat cells with CD3e antibody (clone UCHT1, green) and Reddot nuclear stain (red).

Flow cytometry testing of human Jurkat cells with CD3e antibody (clone UCHT1); Red = isotype control, Blue = CD3e antibody.

SDS-PAGE analysis of purified, BSA-free CD3e antibody (clone UCHT1) as confirmation of integrity and purity.

Quick Database Links

UniProt

P07766