CD3 epsilon Antibody

SKU: orb750028

Description

The CD3 complex mediates signal transduction, resulting in T-cell activation and proliferation. It is required for normal immune responses. This antibody is specific to the epsilon chain of CD3.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Immunology,Epigenetics

Images & Validation

• Tested Applications: FACS, WB
• Dilution Range: Western blot: 1-2ug/ml for 60 min at RT,Flow cytometry: 1-2ug/million cells
• Reactivity: Human
• Application Notes:
  Optimal dilution of the CD3e antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: C3e/1308
• Immunogen: Amino acids 23-119 from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store the CD3e antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This CD3e antibody is available for research use only.
• Disclaimer: For research use only

Western blot testing of human Jurkat cell lysate with CD3e antibody (clone C3e/1308). Predicted molecular weight ~23 kDa.

Western blot testing of 1) a partial recombinant protein and 2) human Jurkat cell lysate with CD3e antibody (clone C3e/1308). Predicted molecular weight ~23 kDa.

SDS-PAGE Analysis of Purified, BSA-Free CD3e Antibody (clone C3e/1308). Confirmation of Integrity and Purity of the Antibody.

Flow cytometry testing of human Jurkat cells with CD3e antibody (clone C3e/1308); Red = isotype control, Blue = CD3e antibody.

Quick Database Links

UniProt

P07766