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CD3 epsilon&CD3 gamma Heterodimer Recombinant Protein

SKU: orb1231144

Description

CD3 epsilon&CD3 gamma Heterodimer Recombinant Protein

Research Area

,Immunology

Images & Validation

Tested ApplicationsELISA, WB
Application Notes
Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 39.2 kDa (CD3E) and 38.6 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus, followed by a polyhist

Key Properties

SourceHEK293 cells
TargetCD3E
TagHis Tag & Fc Tag & Flag Tag
MW39.2 kDa (CD3E) & 38.6 kDa (CD3G)
Purity> 95% as determined by SDS-PAGE.
Protein SequenceAsp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G)

Storage & Handling

StorageLyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles.
Form/AppearanceLyophilized
Buffer/PreservativesPBS, pH 7.4
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CD3 epsilon & CD3 gamma,CD3E & CD3G

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CD3 epsilon&CD3 gamma Heterodimer Recombinant Protein

Human CD3E&CD3G Heterodimer Protein, Fc, His Tag&Fc, Flag Tag (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

CD3 epsilon&CD3 gamma Heterodimer Recombinant Protein

Immobilized Human CD3E&CD3G Heterodimer Protein, Fc, His Tag&Fc, Flag Tag at 2 ug/ml (100 uL/well) can bind UCHT1 with a linear range of 0.4-6 ng/mL.

CD3 epsilon&CD3 gamma Heterodimer Recombinant Protein

Immobilized Human CD3E&CD3G Heterodimer Protein, Fc, His Tag&Fc, Flag Tag at 1 ug/ml (100 uL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3)

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Datasheet
Product Information
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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

CD3 epsilon&CD3 gamma Heterodimer Recombinant Protein (orb1231144)

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0.05 mg
$ 1,020.00
DispatchUsually dispatched within 5-10 working days
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