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Description
Research Area
,Product Categories/Antibodies,Immune cell / T cell marker
Images & Validation
−Item 1 of 8
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot 1:500-1:1000 Immunofluorescence 1:300-1:400 Immunohistochemistry (Paraffin) 1:100-1:300 |
| Reactivity | Human, Mouse, Rabbit |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / Intracellular domain corresponding to Human/mouse CD3 |
| Target | CD3 Intracellular domain |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
|---|---|
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Anti-CD3 antibody at 1/1000 dilution. Lysates at 40 μg per lane. This blot was produced using a 15% SDS-PAGE. NC membrane was then blocked for an hour before being incubated with orb1294242 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded mouse spleen labeling CD3 with orb1294242 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded mouse spleen labeling CD3 with orb1294242 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded mouse spleen labeling CD3 with orb1294242 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded Human Tonsil carcinoma tissue labeling CD3 with orb1294242 at 1/200.
Immunohistochemical analysis of paraffin embedded Human Hepatocellular cancer tissue labeling CD3 with orb1294242 at 1/100.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294242 anti-CD3 antibody at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Product: CD3 antibody; Catalog: orb1294242; Sample: Human peripheral blood; anti-CD3 antibody at 1:100.
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Gene Symbol
CD3 Intracellular domain
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
CD3 Intracellular domain (orb1294242)
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