CD3 (mouse specific), monoclonal

SKU: orb1294281

This product can be custom made

Description

Rat Monoclonal antibody to CD3 (mouse specific)

Research Area

,Product Categories/Antibodies,Immune cell / T cell marker

Images & Validation

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Item 1 of 3

Tested Applications	FC, IF, IHC
Dilution Range	Immunofluorescence 1:100-1:200 Immunohistochemistry (Frozen) 1:50-1:100 Flow Cytometry 1:50-1:100
Reactivity	Mouse

Key Properties

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Host	Rat
Clonality	Monoclonal
Isotype	Rat IgG2a
Immunogen	Synthetic peptide / Extracellular domain corresponding to Mouse CD3
Target	CD3 (mouse specific), monoclonal
Conjugation	Unconjugated

Storage & Handling

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Storage	Phosphate-buffered solution, pH 7.2, containing 0.025% ProClin 300 , 20% Glycerol. Store at -20°C.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Quality Guarantee

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Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294281 Anti-CD3 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Product: CD3 antibody; Catalog: orb1294281; Sample: C57BL/6 mouse splenocytes.

Immunohistochemical analysis of paraffin embedded mouse spleen tissue labeling CD3 with orb1294281 at 1/50.

Quick Database Links

Gene Symbol

CD3 (mouse specific), monoclonal

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Protocol Information

IHC

Immunohistochemistry

Flow Cytometry

Immunofluorescence