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CD31 Antibody
SKU: orb1255366
Featured
Description
Research Area
,Cancer,Cell Cycle,Immunology,Signal Transduction,Stem Cell
Images & Validation
−Item 1 of 4
| Tested Applications | IHC, IP, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 600 to the C-terminus of mouse CD31 (NP_001292086.1). |
| Target | PECAM1 |
| Molecular Weight | Observed: 130kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Platelet endothelial cell adhesion molecule, PECAM-1, CD31, Pecam1, Pecam, Pecam-1
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Western blot analysis of extracts of various cell lines, using CD31 antibody (orb1255366) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3s.
Immunohistochemistry of paraffin-embedded human prostate cancer using CD31 antibody (orb1255366) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using CD31 antibody (orb1255366) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using CD31 antibody (orb1255366) at dilution of 1:100 (40x lens).
Quick Database Links
Gene Symbol
PECAM1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
CD31 Antibody (orb1255366)
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