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Description
Research Area
Images & Validation
−| Tested Applications | FC, IF, IHC-P, WB |
|---|---|
| Reactivity | Human |
| Predicted Reactivity | Monkey |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Immunogen | This CD38 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 241-270 amino acids from the C-terminal region of human CD38. |
| Target | CD38 |
| Molecular Weight | 34 kDa |
| Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−CD38 Recombinant Rabbit Monoclonal Antibody [orb704512]
IF, IHC-Fr, IHC-P, WB
Human
Rabbit
Recombinant
Unconjugated
100 μl, 50 μlCD38 antibody [orb10319]
ICC, IF, IHC-P, WB
Guinea pig, Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μg

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Western blot analysis of lysate from 293T cell line, using CD38 Antibody at 1:1000.

Western blot analysis of lysate from RPMI8226 cell line, using CD38 Antibody at 1:1000.

Formalin-fixed and paraffin-embedded human hepatocarcinoma with CD38 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Immunofluorescence analysis of CD38 Antibody with paraffin-embedded human hepatocarcinoma tissue. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. Red counterstaining is PI.

Flow cytometric analysis of HepG2 cells using CD38 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of CD38 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot analysis of lysate from RPMI 8226 cell line, using CD38 Antibody at 1:1000.
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Protocol Information
CD38 Antibody (orb1264538)
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