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CD3D & CD3E Heterodimer Protein, Mouse, Recombinant (Fc-Flag & Fc-His)

SKU: orb1957209

Description

CD3D & CD3E Heterodimer Protein, Mouse, Recombinant (Fc-Flag & Fc-His) is expressed in HEK293 mammalian cells with Fc-Flag, Fc-His tag. The predicted molecular weight is 76 kDa and the accession number is P04235&P22646.

Research Area

,Recombinant-Protein

Images & Validation

Application Notes
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

Key Properties

Expression SystemHEK293 Cells
Biological OriginMouse
Biological ActivityCD3D & CD3E Heterodimer Protein, Mouse, Recombinant (Fc-Flag & Fc-His) is expressed in HEK293 mammalian cells with Fc-Flag, Fc-His tag. The predicted molecular weight is 76 kDa and the accession number is P04235&P22646.
TagC-hFc-Flag, C-hFc-His
Expression RegionA DNA sequence encoding the extracellular domain (Met 1-Ala 105) of mouse CD3D (P04235) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asp 108) of mouse CD3E (P22646) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD3D/CD3E heterodimer was purified. Predicted N terminal: Phe 22 & Asp 22
MW76 kDa (predicted); 45-50 kDa (reducing condition, due to glycosylation)
Purity98.00%

Storage & Handling

Storage-20°C
Expiration Date6 months from date of receipt.
DisclaimerFor research use only
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UniProt Details

No UniProt data available

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
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50 μg
$ 460.00
DispatchUsually dispatched within 5-10 working days
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