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CD3D & CD3E Heterodimer Protein, Mouse, Recombinant (Fc-Flag & Fc-His)
SKU: orb1957209
Description
Research Area
,Recombinant-Protein
Images & Validation
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| Application Notes |
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Key Properties
−| Expression System | HEK293 Cells |
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| Biological Origin | Mouse |
| Biological Activity | CD3D & CD3E Heterodimer Protein, Mouse, Recombinant (Fc-Flag & Fc-His) is expressed in HEK293 mammalian cells with Fc-Flag, Fc-His tag. The predicted molecular weight is 76 kDa and the accession number is P04235&P22646. |
| Tag | C-hFc-Flag, C-hFc-His |
| Expression Region | A DNA sequence encoding the extracellular domain (Met 1-Ala 105) of mouse CD3D (P04235) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asp 108) of mouse CD3E (P22646) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD3D/CD3E heterodimer was purified. Predicted N terminal: Phe 22 & Asp 22 |
| MW | 76 kDa (predicted); 45-50 kDa (reducing condition, due to glycosylation) |
| Purity | 98.00% |
Storage & Handling
−| Storage | -20°C |
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| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |

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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide