CD3e Antibody

SKU: orb1252812

Description

CD3e Antibody

Research Area

,Cancer,Immunology,Signal Transduction

Images & Validation

• Tested Applications: FC, IP, WB
• Reactivity: Bovine, Canine, Elephant, Equine, Feline, Frog, Gallus, Guinea pig, Hamster, Human, Mole Rat, Mouse, Opossum, Porcine, Primate, Rabbit, Rat, Sheep
• Application Notes:
  Flow Cytometry: use the CD3e antibody at 0.5-1 ug/million cellsWB: 0.5-1.0 ug/mlIP: 1-2ug/500ug protein lysateThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD3e antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Amino acids ERPPPVPNPDYEP (156-168) were used as the immunogen for this CD3e antibody.
• Target: CD3E
• Purification: Protein A purified CD3e antibody
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E

Western blot testing of human Jurkat lysate in the absence and presence of immunizing peptide, and mouse thymus lysate probed with CD3e antibody.

Western blot testing of Jurkat lysate with CD3e antibody.

Quick Database Links

Gene Symbol

CD3E

UniProt

P07766