CD3e Antibody [PC3/188A]

SKU: orb1252166

Description

CD3e Antibody [PC3/188A]

Research Area

,Cancer,Immunology,Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Bovine, Equine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat
• Application Notes:
  WB: 1-2 ug/mlIHC (FFPE): 1-2 ug/ml for 30 min at RTFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlOptimal dilution of the CD3e antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: PC3/188A
• Immunogen: Amino acids 156-168 (cytoplasmic domain) from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.
• Target: CD3E
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E

IHC testing of human tonsil tissue with CD3e antibody (clone PC3/188A). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

Western blot testing of human Jurkat cell lysate with CD3e antibody (clone PC3/188A). Expected molecular weight ~23 kDa.

SDS-PAGE Analysis of Purified, BSA-Free CD3e Antibody (clone PC3/188A). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

CD3E

UniProt

P07766