CD3E & CD3G Heterodimer Protein, Human, Recombinant (Fc-Flag & Fc-His)

SKU: orb1953120

Description

CD3E & CD3G Heterodimer Protein, Human, Recombinant (Fc-Flag & Fc-His) is expressed in HEK293 mammalian cells with Fc-Flag, Fc-His tag. The predicted molecular weight is 78.36 kDa and the accession number is NP_000724.1.

Research Area

,Recombinant-Protein

Images & Validation

• Application Notes:
  A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

Key Properties

• Expression System: HEK293 Cells
• Biological Origin: Human
• Biological Activity: CD3E & CD3G Heterodimer Protein, Human, Recombinant (Fc-Flag & Fc-His) is expressed in HEK293 mammalian cells with Fc-Flag, Fc-His tag. The predicted molecular weight is 78.36 kDa and the accession number is NP_000724.1.
• Tag: C-hFc-Flag, C-hFc-His
• Expression Region: A DNA sequence encoding the (Met1-Asp126) of human CD3E (NP_000724.1) was fused with a C-terminal Fc region of human IgG1 tag followed by a FLAG tag, constructed the plasmid 1; A DNA sequence encoding the (Met1-Ser116) of human CD3G (NP_000064.1) was fused with a C-terminal Fc region of human IgG1 tag followed by a polyhistidine tag, constructed the plasmid 2. The two plasmids were co-expressed and the Human CD3E/CD3G heterodimer was purified. Predicted N terminal: Asp 23 & Gln 23
• MW: 78.36 kDa (predicted); 47.10 and 42.93 kDa (reducing condition, due to glycosylation)
• Purity: 98.00%

Storage & Handling

• Storage: -20°C
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

P09693, P07766