CD3E & CD3G Heterodimer Protein, Human, Recombinant (Fc-Flag & mFc)

SKU: orb1955714

Description

CD3E & CD3G Heterodimer Protein, Human, Recombinant (Fc-Flag & mFc) is expressed in HEK293 mammalian cells with Fc-Flag, mFc tag. The predicted molecular weight is 76.9 kDa and the accession number is NP_000724.1&NP_000064.1.

Research Area

,Recombinant-Protein

Images & Validation

• Application Notes:
  A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

Key Properties

• Expression System: HEK293 Cells
• Biological Origin: Human
• Biological Activity: CD3E & CD3G Heterodimer Protein, Human, Recombinant (Fc-Flag & mFc) is expressed in HEK293 mammalian cells with Fc-Flag, mFc tag. The predicted molecular weight is 76.9 kDa and the accession number is NP_000724.1&NP_000064.1.
• Tag: C-hFc-Flag, C-mFc
• Expression Region: A DNA sequence encoding the extracellular domain (Met1-Asp126) of human CD3E (NP_000724.1) was fused with the C-terminal FLAG-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Ser116) of human CD3G (NP_000064.1) was fused the Fc region of mouse IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3E/CD3G heterodimer was purified. Predicted N terminal: Asp 23 & Gln 23
• MW: 76.9 kDa (predicted); 42-47 kDa (reducing condition, due to glycosylation)
• Purity: 94.60%

Storage & Handling

• Storage: -20°C
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

NP_000724.1&NP_000064.1