CD3E Rabbit Polyclonal Antibody

SKU: orb1175

Description

CD3E Rabbit Polyclonal Antibody

Research Area

CD Markers, Cell Biology, Human Lineage-negative, Stem Cell Research, Immunology

Images & Validation

• Tested Applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse CD3 epsilon
• Target: CD3E
• Molecular Weight: 20 kDa
• Purification: affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD3 epsilon; CD3e; CD3e antigen epsilon polypeptide(TiT3 complex); CD3E antigen epsilon polypeptide; CD3E antigen, epsilon subunit; CD3e molecule epsilon; CD3e molecule, epsilon(CD3 TCR complex); CD3e molecule, epsilon(CD3-TCR complex); CD3E_HUMAN; CD3E_MOUSE; IMD18; T cell antigen receptor complex epsilon subunit of T3; T cell surface antigen T3/Leu 4 epsilon chain; T cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE.

Sample: Lane 1: Human MOLT4 cell lysates, Lane 2: Human Jurkat cell lysates, Primary: Anti-CD3E (orb1175) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 20 kDa, Observed band size: 20 kDa.

Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-CD3E (orb1175) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 20 kD, Observed band size: 20 kD.

Quick Database Links

Gene Symbol

CD3E

UniProt

P22646