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CD3e (T-Cell Marker) Antibody

SKU: orb2313723

Description

CD3e (T-Cell Marker) Antibody

Research Area

,Cardiovascular|Hematopoietic Stem Cells|Immunology|PD-1 blockade immunotherapy

Images & Validation

Tested ApplicationsELISA, FC, IF, IHC
Dilution RangeELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA); ,Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml). ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
ReactivityHuman, Primate
Application Notes
Positive Control: Human peripheral blood. Jurkat cells. Human tonsil or lymph node

Key Properties

HostMouse
ClonalityMonoclonal
IsotypeIgG1
Clone No.UCHT1
ImmunogenHuman infant thymocytes and peripheral blood lymphocytes from a S zary Syndrome donor
TargetCD3E
Source/Expression SystemBioreactor
Molecular Weight20kDa
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
ConcentrationPurified Ab WITHOUT BSA at 1.0mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CD3 epsilon; CD3 TCR complex; T cell antigen receptor complex epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE; TiT3 complex

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

Protocol Information

IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 660.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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