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CD4 rabbit pAb

SKU: orb764784

Description

Rabbit polyclonal antibody to CD4

Research Area

Product Categories/Antibodies/Primary Antibodies,Product Categories/Small Molecules,Product Categories/Antibodies

Images & Validation

Tested ApplicationsELISA, IF, IHC-P, WB
Dilution RangeWestern Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
ReactivityHuman, Mouse

Key Properties

ClonalityPolyclonal
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human CD4. AA range:401-450
Molecular Weight51
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Anti-CD4 antibody, anti-T-cell surface glycoprotein CD4 antibody, anti-T-cell surface antigen T4/Leu-3 antibody, anti-CD antigen CD4 antibody

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CD4 rabbit pAb

Immunofluorescence analysis of A549. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

CD4 rabbit pAb

Western Blot analysis of various cells using CD4 Polyclonal Antibody diluted at 1:500.

CD4 rabbit pAb

Western Blot analysis of COLO205 cells using CD4 Polyclonal Antibody diluted at 1:500.

CD4 rabbit pAb

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive control (right) obtaned from antibody was pre-absorbed by immunogen peptide.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

CD4 rabbit pAb (orb764784)

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Available Sizes

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50 μl
$ 250.00
100 μl
$ 350.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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