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Description
Research Area
,Recombinant-Protein
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Expression System | HEK293 Cells |
|---|---|
| Biological Origin | Mouse |
| Biological Activity | CD40 Ligand Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 44.4 kDa and the accession number is P27548. |
| Tag | N-hFc |
| Expression Region | A DNA sequence encoding the soluble form of mouse CD40LG (P27548) (Gly 115-Leu 260) was fused with the Fc region of human IgG1 at the N-terminus. Predicted N terminal: Glu |
| MW | 44.4 kDa (predicted); 50 kDa (reducing conditions) |
| Purity | 98.00% |
Storage & Handling
−| Storage | -20°C |
|---|---|
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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Mouse CD40 Ligand Protein, hFc Tag [orb1290850]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 42.1 kDa after removal of the signal peptide. The apparent molecular mass of hFc-CD40L is approximately 40-55 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μg CD40 Ligand Protein, Mouse, Recombinant (monomeric hFc) [orb1960052]
98.00%
75.6 kDa (predicted). Due to glycosylation, the protein migrates to 76-80 kDa based on Tris-Bis PAGE result.
500 μg, 100 μg, 1 mg
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Protocol Information
Protein Handling and Storage Guide
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