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Description
Research Area
,Apoptosis,Immunology,Signal Transduction
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Recombinant CD40L is fully biologically active when compared to standards.ED50 is 5-10 ng/ml as determined by the dose-dependent stimulation of IL-12 & IL-8 induction by PMB (Peripheral Mononuclear) cells. |
| Target | CD40LG |
| Tag | Tag Free |
| MW | 16.2 kD |
| Purity | Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
| Protein Sequence | Met113-Leu261 |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20˚°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7˚°C for 2-7 days.Aliquots of reconstituted samples are stable at -20˚°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Lyophilized from a 0.2 um filtered solution of 20mM PB, 200mM NaCl, 0.1mM EDTA, pH 7.0. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD40 Ligand, CD40-L, T-Cell Antigen Gp39, TNF-Related Activation Protein, TRAP, Tumor Necrosis Factor Ligand Superfamily Member 5, CD154, CD40LG, CD40L, TNFSF5
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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