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Description
Research Area
,Apoptosis,Immunology,Signal Transduction
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | CHO cells |
|---|---|
| Biological Activity | Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86) (ED50: < 1ng/ml). |
| Target | CD40LG |
| Tag | DDDDK Tag |
| Purity | > 95% (SDS-PAGE). Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza). |
| Protein Sequence | Human CD40L (aa 116-261) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a DDDDK-tag. |
Storage & Handling
−| Storage | Stable for at least 6 months after receipt when stored at -20˚°C. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Contains PBS Reconstitute with 100 μl sterile water. |
| Concentration | 0.1mg/ml after reconstitution. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ACRP30headless:CD40L, ACRP30headless:CD154, ACRP30headless:TNFSF5, ADIPOQ-CD40L
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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