CD44 Antibody / HCAM

SKU: orb640057

Description

Recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. This MAb selectively interferes with lymphocyte binding to lymph node, mucosal and synovial endothelium. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

Research Area

Product Categories/Products/Antibodies,Product Categories/Products/Antibodies/Primary Antibodies

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: Mouse IgG1, kappa
• Clone No.: rHCAM/918
• Immunogen: A recombinant full-length human CD44 protein was used as the immunogen for this recombinant CD44 antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store the recombinant CD44 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This recombinant CD44 antibody is available for research use only.
• Disclaimer: For research use only