CD44 Antibody

SKU: orb1333592

Description

Mouse monoclonal antibody to CD44

Research Area

Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: FC, IHC, WB
• Dilution Range: WB 1:400~8000, IHC 1:150, IF 1:100, FLOW 1:50
• Reactivity: Human
• Application Notes:
  For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: OTI1E3
• Immunogen: Full length human recombinant protein of human CD44(NP_000601) produced in HEK293T cell.
• Target: CD44
• Molecular Weight: 79.2 kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD44 (RC221771, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD44. P

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD44 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CD44 mouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD44 mouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CD44 mouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CD44 mouse monoclonal antibody.

Anti-CD44 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD44 (RC221771).

Flow cytometric Analysis of living Colo205 cells, using anti-CD44 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Quick Database Links

Gene Symbol

CD44

UniProt

P16070

RefSeq (mRNA):NM_000610