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Description
Research Area
,Recombinant-Protein
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Expression System | HEK293 Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | CD44 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 23.4 kDa and the accession number is P16070-1. |
| Tag | C-His |
| Expression Region | A DNA sequence encoding the N-terminal fragment (Met 1-Pro 220) of human CD44 (P16070-1) extracellular domain was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Gln 21 |
| MW | 23.4 kDa (predicted); 43-48 kDa (reducing condition, due to glycosylation) |
| Purity | 95.10% |
Storage & Handling
−| Storage | -20°C |
|---|---|
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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Human CD44 Protein, His Tag [orb757431]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 22.9 kDa after removal of the signal peptide.The apparent molecular mass of CD44-His is approximately 35-55 kDa due to glycosylation.
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80.2 kDa (predicted)
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> 99.9%
46 KDa (reducing condition)
1 mg, 500 μg, 50 μg, 10 μgPCBP1 Protein, Human, Recombinant (His) [orb1958052]
> 87 % as determined by SDS-PAGE
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Protocol Information
Protein Handling and Storage Guide
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