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Research Area
Product Categories/Products/Antibodies,Product Categories/Research Area,Neuroscience,Epigenetics,Infectious Diseases,Primary Antibodies;Cancer;Immunology;Neuroscience;Signal Transduction;Fc gamma R-mediated phagocytosis;T cell activation;T cell receptor signaling;JAK-STAT signaling;SARS-Human interaction partners
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| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:1000, IHC-P: 1:10-50, FACS: 1:10-50 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB00430 |
| Immunogen | Synthetic Peptide |
| Target | PTPRC |
| Molecular Weight | 147486 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti CD 45 antibody, anti CD45 antibody, anti CD45 antigen antibody, anti CD45R antibody, anti L CA antibody, anti L-CA antibody, anti LCA antibody, anti Leukocyte common antigen antibody, anti loc antibody, anti Ly-5 antibody, anti LY5 antibody, anti Ly5, homolog of antibody, anti Lyt-4 antibody, anti Protein tyrosine phosphatase receptor type C antibody, anti Protein tyrosine phosphatase receptor type c polypeptide antibody, anti protein tyrosine phosphatase, receptor type, C antibody, anti PTPRC antibody, anti Receptor-type tyrosine-protein phosphatase C antibody, anti SCID due to PTPRC deficiency antibody, anti T200 antibody, anti T200 glycoprotein antibody, anti T200 leukocyte common antigen antibody
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The anti-CD45 (C-term) Pab is used in Western blot to detect CD45 in jurkat cell lysate.
Flow cytometric analysis of jurkat cells using CD45 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human tonsil reacted with CD45 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_002829.3, NP_563578.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
CD45 Antibody (C-term) (orb33270)
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