CD45 / LCA (B-Cell Marker) Antibody

SKU: orb2317635

Description

CD45 / LCA (B-Cell Marker) Antibody

Research Area

,Autophagy|B Cell Markers|Cancer|Dendritic Cell Marker|Developmental Biology|Hematopoietic Stem Cells|Immuno Oncology|Immunology|Mesenchymal Stem Cell Differentiation

Images & Validation

• Tested Applications: FC, IF, IHC
• Dilution Range: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: Ramos, U-698, or GA-10 cells. Tonsil

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: PTPRC/1460
• Immunogen: Human B-lymphocytes
• Target: PTPRC
• Source/Expression System: HEK293 cells
• Molecular Weight: 180-220kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein

Quick Database Links

Gene Symbol

PTPRC

UniProt

P08575

NCBI Gene ID

5788