CD45RA Antibody (Leukocyte marker)

SKU: orb2638190

Description

CD45RA is an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Due to variation in protocol and secondary antibody used, the CD45RA antibody may need to be titered for optimal performance.1. FFPE staining requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: 111-1C5
• Immunogen: Stimulated human leukocytes were used as immunogen for this CD45RA antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This CD45RA antibody is available for research use only.
• Disclaimer: For research use only

Immunofluorescent staining of PFA-fixed human Raji cells with CD45RA antibody (clone 111-1C5, green) and Reddot nuclear stain (red).

IHC staining of FFPE human tonsil tissue with CD45RA antibody (clone 111-1C5). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human spleen tissue lysate with CD45RA antibody. Expected molecular weight: 147-220 kDa depending on glycosylation level.

Flow cytometry testing of PFA-fixed human Raji cells with anti-CD45RA antibody (clone 111-1C5); Red = isotype control, Blue = anti-CD45RA antibody.

SDS-PAGE analysis of purified, BSA-free CD45RA antibody (clone 111-1C5) as confirmation of integrity and purity.

Quick Database Links

UniProt

P08575