CD45RO (T-Cell Marker) Antibody

SKU: orb2320187

Description

CD45RO (T-Cell Marker) Antibody

Research Area

,Autophagy|B Cell Markers|Cancer|Dendritic Cell Marker|Developmental Biology|Hematopoietic Stem Cells|Immuno Oncology|Immunology|Mesenchymal Stem Cell Differentiation

Images & Validation

• Tested Applications: FC, IF, IHC
• Dilution Range: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Reactivity: Human, Monkey, Primate
• Application Notes:
  Positive Control: CCRF-CEM, Jurkat or MOLT-4 cells. Paracortex in a tonsil or a reactive lymph node.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: UCHL-1
• Immunogen: Cultured human T-cells from an IL-2-dependent T-cell line (CA1)
• Target: PTPRC
• Source/Expression System: Bioreactor
• Molecular Weight: 180-185kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

T200, GP180, LCA, Leukocyte Common Antigen, LY5, Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein

Quick Database Links

Gene Symbol

PTPRC

UniProt

P08575

NCBI Gene ID

5788