CD63 Antibody (C-term)

SKU: orb35630

Description

Rabbit polyclonal antibody to CD63 (also named as LAMP-3, Melanoma-associated antigen ME491, TSPAN30, MLA1 and OMA81H) is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 antigen has a diverse distribution on the surface and in the cytoplasm of many cell types including lymphoid, myeloid, endothelial cells and melanoma.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Epigenetics,Primary Antibodies;Cancer;Cardiovascular;Immunology;Lysosome

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: IHC-P: 1:50-100, FACS: 1:25, WB: 1:1000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB26813
• Immunogen: Synthetic Peptide
• Target: CD63
• Molecular Weight: 25637
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-CD_antigen=CD63 antibody, anti-Lysosomal-associated membrane protein 3 antibody, anti-LAMP-3 antibody, anti-Tetraspanin-30 antibody, anti-Tspan-30 antibody, anti-Granulophysin antibody, anti-OMA81H antibody, anti-Ocular melanoma-associated antigen antibody, anti-Melanoma-associated antigen ME491 antibody, anti-CD63 antigen, TSPAN30 antibody, anti-MLA1 antibody, anti-CD63 antibody

Anti-CD63 Antibody (C-term) at 1:2000 dilution + human plasma lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 25 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-CD63 Antibody (C-term) at 1:1000 dilution + HL-60 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40-50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CD63 Antibody (C-term) at 1:1000 dilution. Lane 1: A2058 whole cell lysates. Lane 2: MDA-MB-453 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 26 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CD63 Antibody (C-term) at 1:2000 dilution. Lane 1: MDA-MB-453 whole cell lysate. Lane 2: A2058 whole cell lysate. Lane 3: A375 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40-50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CD63 Antibody (C-term) at 1:2000 dilution. Lane 1: MDA-MB-453 whole cell lysate. Lane 2: A2058 whole cell lysate. Lane 3: A375 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40-50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CD63 Antibody (C-term) at 1:2000 dilution. Lane 1: HL-60 whole cell lysate. Lane 2: U-251 MG whole cell lysate. Lane 3: THP-1 whole cell lysate. Lane 4: A2058 whole cell lysate. Lane 5: A375 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40-50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CD63 Antibody (C-term) at 1:2000 dilution. Lane 1: A2058 whole cell lysates. Lane 2: A375 whole cell lysates. Lane 3: HL-60 whole cell lysates. Lane 4: THP-1 whole cell lysates. Lane 5: U-87 MG whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 25 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

CD63

UniProt

P08962

RefSeq (Protein):NP_001244329.1, NP_001244319.1, NP_001244330.1, NP_001254627.1, NP_001244321.1, NP_001771.1, NP_001244318.1, NP_001244320.1