CD71 Antibody (C-term)

SKU: orb34369

Description

Rabbit polyclonal antibody to CD71

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Infectious Diseases,Primary Antibodies;Cancer;Cardiovascular;Immunology;Metabolism;Signal Transduction;Catalog Updated;Host cell receptor for virus entry;Viral entry into host cell;Host-virus interaction;SARS-Human interaction partners

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: IHC-P: 1:10-50, WB: 1:1000, FACS: 1:10-50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB20736
• Immunogen: Synthetic Peptide
• Target: TFRC
• Molecular Weight: 84871
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CD71 antibody, anti CD71 antigen antibody, anti Mtvr-1 antibody, anti Mtvr1 antibody, anti p90 antibody, anti sTfR antibody, anti T9 antibody, anti TFR antibody, anti TFR1 antibody, anti TFRC antibody, anti TFRC transferrin receptor (p90 CD71) antibody, anti TR antibody, anti transferrin receptor antibody, anti transferrin receptor protein 1 antibody, anti Transferrin receptor protein 1, serum form antibody, anti TRFR antibody

Western blot analysis of CD71 Antibody (C-term) in Hela cell line lysates (35 ug/lane). CD71 (arrow) was detected using the purified Pab.

Flow cytometric analysis of hela cells using CD71 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram) FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human brain tissue reacted with CD71 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-CD71 Antibody (C-term) at 1:2000 dilution. Lane 1: A549 whole cell lysate. Lane 2: CCRF-CEM whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 85 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining CD71 in human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Quick Database Links

Gene Symbol

TFRC

UniProt

P02786

RefSeq (Protein):NP_001121620.1, NP_003225.2