CD73 Antibody

SKU: orb750319

Description

Mouse monoclonal antibody against CD73 conjugated to Biotin

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Epigenetics

Images & Validation

• Tested Applications: ELISA, FACS, IF, IHC-P, WB
• Dilution Range: ELISA: order Ab without BSA for coating,Flow cytometry: 2-4ug/million cells in 0.1ml,Immunofluorescence: 2-4ug/ml,Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 minutes at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the CD73 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: NT5E/2646
• Immunogen: Recombinant full-length human NT5E protein was used as the immunogen for the CD73 antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Biotin

Storage & Handling

• Storage: Store the CD73 antibody at 2-8°C (up to one month) or aliquot and store at -20°C (longer term).
• Buffer/Preservatives: 0.1 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This CD73 antibody is available for research use only.
• Disclaimer: For research use only

IHC staining of FFPE human tonsil tissue with biotinylated CD73 antibody (clone NT5E/2646). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with biotinylated CD73 antibody (clone NT5E/2646). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using CD73 antibody (clone NT5E/2646). These results demonstrate the foremost specificity of the NT5E/2646 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free CD73 antibody (clone NT5E/2646) as confirmation of integrity and purity.

Quick Database Links

UniProt

P21589