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Description
Research Area
,Cancer,Cell Cycle,Chemokines & Cytokines,Immunology,Innate Immunity,Signal Transduction
Images & Validation
−Item 1 of 1
| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human B7-1, Fc Tag (HPLC-verified) at 2 ug/mL (100 ul/well) can bind Biotinylated Human CTLA-4 with a linear range of 50 - 500 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Human B7-1, Fc Tag (HPLC-verified) at 2 ug/mL (100 ul/well) can bind Human CTLA-4, Strep Tag with a linear range of 1-20 ng/mL. |
| Target | CD80 |
| Tag | C-Fc Tag |
| MW | 50 kDa |
| Purity | > 95% as determined by SEC-HPLC and SDS-PAGE. |
| Protein Sequence | Val 35 - Asn 242 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 50 mM tris, 100 mM glycine, pH 7.5 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD80, B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7
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Human B7-1, Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_005182.1 |
|---|
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
CD80 Recombinant Protein (orb1228180)
- 0.0
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