CD8A Antibody (N-term)

SKU: orb1936090

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

,Primary Antibodies;Cancer;Immunology;Signal Transduction;Immunity;T cell receptor signaling

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB13990
• Target: This CD8A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-88 amino acids from the N-terminal region of human CD8A.
• Molecular Weight: 25729 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a, CD8A, MAL

Western blot analysis of anti-CD8A Antibody (N-term) in Hela cell line lysates (35 ug/lane). CD8A (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human tonsil tissue reacted with CD8A Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-CD8A Antibody (N-term) at 1:1000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: MOLT-4 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 26 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

This CD8A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-88 amino acids from the N-terminal region of human CD8A.

UniProt

P01732

RefSeq (Protein):NP_001759.3, NP_741969.1, NP_001139345.1