CD8A Antibody

SKU: orb1261133

Description

CD8A Antibody

Research Area

,Immunology,Stem Cell

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-182 of human CD8A (NP_001759.3).
• Target: CD8A
• Molecular Weight: Observed: 30kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a, CD8A, MAL

Western blot analysis of extracts of mouse pancreas, using CD8A antibody (orb1261133) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human tonsil using CD8A antibody (orb1261133) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using CD8A antibody (orb1261133) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using CD8A antibody (orb1261133) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using CD8A antibody (orb1261133) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC12 cells using CD8A antibody (orb1261133) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

CD8A

UniProt

P01732