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Description
Research Area
,AKT Signaling|Apoptosis|Autophagy|Cardiovascular|Complement System|Endothelial Cell Marker|MAPK Signaling|Signal Transduction|Transcription Factors
Images & Validation
−
| Tested Applications | ELISA, IHC |
|---|---|
| Dilution Range | ELISA (For coating, order Ab without BSA); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone No. | FAS/3112 |
| Immunogen | Recombinant fragment (around aa26-96) of human CD95 (FAS) protein (exact sequence is proprietary) |
| Target | FAS |
| Source/Expression System | Bioreactor |
| Molecular Weight | 38-50kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Concentration | Purified Ab with BSA and Azide at 200ug/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ALPS1A, Apoptosis APO1 antigen 1, Apoptosis-mediating surface antigen FAS, APT1, Delta Fas, Fas AMA, Fas cell surface death receptor, FAS1, FASL receptor, FASLG receptor, FASTM, Surface antigen APO1, Tumor necrosis factor receptor superfamily member 6 (TNFRSF6)
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Protocol Information
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)