CDC2 monoclonal antibody (M01), clone 1A4-1A9

SKU: orb2294712

Description

Mouse monoclonal antibody raised against a full length recombinant CDC2.

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 Kappa
• Clone No.: 1A4-1A9
• Immunogen: CDC2 (AAH14563, 1 a.a. ~ 297 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRHPNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Detection limit for recombinant GST tagged CDC2 is approximately 30 ng/ml as a capture antibody.

CDC2 monoclonal antibody (M01), clone 1A4-1A9 Western Blot analysis of CDC2 expression in Hela.

CDC2 monoclonal antibody (M01), clone 1A4-1A9. Western Blot analysis of CDC2 expression in 293.

CDC2 monoclonal antibody (M01), clone 1A4-1A9. Western Blot analysis of CDC2 expression in A-549.

CDC2 monoclonal antibody (M01), clone 1A4-1A9. Western Blot analysis of CDC2 expression in HL-60.

CDC2 monoclonal antibody (M01), clone 1A4-1A9. Western Blot analysis of CDC2 expression in Jurkat.

CDC2 monoclonal antibody (M01), clone 1A4-1A9. Western Blot analysis of CDC2 expression in MCF-7.

CDC2 monoclonal antibody (M01), clone 1A4-1A9. Western Blot analysis of CDC2 expression in Raji.

Immunofluorescence of monoclonal antibody to CDC2 on HeLa cell. [antibody concentration 10 ug/ml].

Immunoperoxidase of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 0.3 ug/ml].

Lane 1: CDC2 monoclonal antibody (M01)(Cat # orb2294712), clone 1A4-1A9. Western Blot analysis of CDC2 expression in NIH/3T3 (34.095 KDa). Lane 2: ACTB monoclonal antibody(M01)(Cat # orb2296444), clone 3G4-F9 Western Blot in NIH/3T3 (41.73kDa) used as specificity and loading control.

Western Blot analysis of CDC2 expression in recombinant protein by CDC2 monoclonal antibody (M01), clone 1A4-1A9. Lane 1: CDC2 Protein (0.2 ug). Lane 2: CDK2 Protein (0.2 ug). Lane 3: CDK3 Protein (0.2 ug). Lane 4: CDK4 Protein (0.2 ug). Lane 5: CDK5 Protein (0.2 ug). Lane 6: CDK6 Protein (0.2 ug).

Western Blot analysis of CDC2 expression in transfected 293T cell line by CDC2 monoclonal antibody (M01), clone 1A4-1A9. Lane 1: CDC2 transfected lysate(34.1 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (58.41 KDa).

Quick Database Links

RefSeq:AAH14563