CDC37 Antibody: APC

SKU: orb151597

Description

Rabbit polyclonal to CDC37 (APC). Hsp90 co-chaperone Cdc37 is a protein that is encoded by the CDC37 gene. It has been found to form complexes with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF1, MOK and elF-2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases. CDC37 is necessary for maintaining prostate tumor cell growth and represents a novel target in the exploration for multi-targeted therapies.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Cancer | Heat Shock | Cell Signaling | Epigenetics and Nuclear Signaling

Images & Validation

• Tested Applications: ICC, IF, IHC
• Dilution Range: WB (1:2000), ICC/IF (1:200)
• Reactivity: Human
• Application Notes:
  A 1:2000 dilution of SPC-142 was sufficient for detection of cdc37 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Native human Cdc37, full length
• Target: CDC37
• Molecular Weight: 44.5kDa
• Conjugation: APC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1.68 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Hsp90 co chaperone Cdc37 antibody, CDC 37 antibody, Cdc37 antibody, CDC37 cell division cycle 37 homolog antibody, CDC37 cell division cycle 37 S cerevisiae homolog antibody, CDC37 cell division cycle 37 S cerevisiae homolog of antibody, Cdc37 homolog antibody, CDC37 protein antibody, CDC37_HUMAN antibody, CDC37A antibody, cell division cycle 37 antibody, Cell division cycle 37 homolog antibody, Hsp90 chaperone protein kinase targeting subunit antibody, Hsp90 chaperone protein kinase targeting subunit p50Cdc37 antibody, Hsp90 chaperone protein kinase-targeting subunit antibody, Hsp90 co-chaperone Cdc37 antibody, p50 antibody, p50Cdc37 antibody, S cerevisiae hypothetical protein CDC37 antibody

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-CDC37 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-CDC37 Polyclonal Antibody at 1:200 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-CDC37 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.

Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of CDC37 protein using Rabbit Anti-CDC37 Polyclonal Antibody. Primary Antibody: Rabbit Anti-CDC37 Polyclonal Antibody at 1:2000.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-CDC37 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-CDC37 Polyclonal Antibody at 1:200 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-CDC37 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.

Quick Database Links

Gene Symbol

CDC37

UniProt

Q16543

NCBI Gene ID

11140

RefSeq (Protein):NP_008996.1