CENP-A Antibody: FITC

SKU: orb148111

Description

Mouse monoclonal to CENP-A (FITC). A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The Centromere Protein-A, CENP-A, is a Histone H3-like protein that contains a C-terminal H3-like domain, which is required for centromere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA CENP-A forms an octa-meric complex with histones H4, H2A, H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome..

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Infectious Diseases,Cell Signaling | Epigenetics and Nuclear Signaling

Images & Validation

• Tested Applications: ICC, IF, IHC
• Dilution Range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Human
• Application Notes:
  1 µg/ml of SMC-202 was sufficient for detection of CENPA in 20 µg of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 5A7-2E11
• Immunogen: Synthetic peptide corresponding to a portion of human CENP-A
• Target: CENPA
• Molecular Weight: 18kDa
• Conjugation: FITC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 640.91mM DMSO, 136.36mM Ethanolamine, 9.09mM Sodium Bicarbonate in 90.9% PBS
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CENP A antibody, cenpa antibody, Centromere auto antigen A antibody, Histone H3 like centromeric protein A antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CENP-A Monoclonal Antibody, Clone 5A7-2E11. Tissue: Colon cancer cell line (HT-29). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.

Western Blot analysis of Human U2OS cell lysate showing detection of CENP-A protein using Mouse Anti-CENP-A Monoclonal Antibody, Clone 5A7-2E11. Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:1000.

Quick Database Links

Gene Symbol

CENPA

UniProt

P49450

NCBI Gene ID

1058

RefSeq (Protein):NP_001035891.1